?EFFECT OF A PROTEASE INHIBITOR ON ADENYLATE CYCLASE OF BORDETELLA PERTUSSIS

Authors

  • YASMEEN FAIZ KAZI Department of Microbiology, Shah Abdul Latif University, Khairpur; Pakistan
  • BADARUDDIN A. MEMON Department of Microbiology, Shah Abdul Latif University, Khairpur; Pakistan

Keywords:

Protease, Bordetella pertussis, Adenylate cycl

Abstract

The heterogeneity of adenylate cyclase (AC) of Bordetella pertussis reported in the literature has been revealed by
SDS-PAGE and immunoblotting. This signifies that AC might be susceptible to endogenous proteases. The multiplicity of
the bands appeared in SDS-PAGE and immunoblotting were found to be reduced when a protease inhibitor, phenyl methyl
sulphonyl flouride (PM SF) was used during AC preparation. This study indicates that AC does not exist physiologically
as multiple peptides.

Published

1997-10-20

Issue

Vol. 40 No. 5-12 (1997): Pakistan Journal of Scientific and Industrial Research

Section

Articles

How to Cite

?EFFECT OF A PROTEASE INHIBITOR ON ADENYLATE CYCLASE OF BORDETELLA PERTUSSIS. (1997). Biological Sciences - PJSIR, 40(5-12), 1-2. https://www.v2.pjsir.org/index.php/biological-sciences/article/view/2348