Presence of Anti-Nuclear and Anti-Double-Stranded DNA Antibodies among Healthy Individuals in the Twin Cities (Rawalpindi and Islamabad) of Pakistan

Seroprevalence of Autoantibodies in Rawalpandi and Islamabad

Authors

  • Muhammad Umer Khan Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
  • Amina shafqat Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
  • Muhammad Usman Ghani Centre for Applied Molecular Biology, University of the Punjab, Lahore, Pakistan
  • Saima Younis Centre for Applied Molecular Biology, University of the Punjab, Lahore, Pakistan
  • Tazeen Zahid Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
  • Iram Amin Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
  • Muhammad Shahid Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
  • Inamullah Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan

Keywords:

anti-nuclear, anti-double-stranded, DNA, antibodies, autoimmunity

Abstract

Background: The study aimed to investigate antinuclear antibodies (ANA) and anti-double- stranded DNA antibodies among healthy individuals in Rawalpindi and Islamabad, emphasizing the need to understand their prevalence in the general population.

Objective: To determine ANA and anti-dsDNA levels, assessing their potential as disease biomarkers in the absence of clinical symptoms among healthy individuals.

Methods: A total of 659 individuals participated in the study, including 503 females (76.3%) and 156 males (23.5%). Blood samples were collected using convenient sampling technique followed by separation of serum using centrifugation. The levels of ANA and anti-dsDNA were quantified using non-competitive ELISA.

Results: The results of the study showed that only 0.91% of individuals tested positive for ANA and 0.46% for anti-dsDNA. This indicates a minimal presence of these antibodies in the healthy cohort. Furthermore, no significant association was found between ANA and anti-dsDNA.

Conclusion: This study highlights a low occurrence of ANA and anti-dsDNA among healthy subjects, suggesting their potential exclusive association with autoimmune diseases.

Downloads

Published

2025-10-24

How to Cite

Muhammad Umer Khan, Amina shafqat, Muhammad Usman Ghani, Saima Younis, Tazeen Zahid, Iram Amin, Muhammad Shahid, & Inamullah. (2025). Presence of Anti-Nuclear and Anti-Double-Stranded DNA Antibodies among Healthy Individuals in the Twin Cities (Rawalpindi and Islamabad) of Pakistan: Seroprevalence of Autoantibodies in Rawalpandi and Islamabad. Biological Sciences - PJSIR, 68(3), 254–261. Retrieved from http://www.v2.pjsir.org/index.php/biological-sciences/article/view/3146

Issue

Vol. 68 No. 3 (2025): Pakistan Journal of Scientific and Industrial Research Series B: Biological Sciences

Section

Articles